Yan L, Bhave M
Centre for Bioprocessing and Food Technology, Victoria University of Technology, P.O. Box 14428, MC, Melbourne 8001, Australia.
Biochem Genet. 2000 Dec;38(11-12):391-411. doi: 10.1023/a:1026436831777.
The waxy proteins from a number of genetic backgrounds of wheat and its progenitors were analyzed by SDS-PAGE. The deduced amino acid sequences of the waxy proteins of three diploid progenitor species indicated several key amino acid substitutions, which could explain the differences observed in the electrophoretic mobilities of the wheat waxy proteins. A slight difference observed in the apparent molecular weight of the WX-A1 protein of diploid and polyploid wheat was explained by amino acid substitutions or variations in predicted protein structures. Further, twelve different partial genomic clones, representing the individual waxy loci of the various diploid, tetraploid and hexaploid wheats, were isolated and compared. The results indicated significant variations in intron 4 and led to identification of sequences unique to the individual waxy genes and genomes of wheat and its proposed progenitors. The sequence variations observed have a great potential for development as molecular markers for identification of specific waxy loci and study of the various waxy mutants of wheat.
通过SDS-PAGE分析了来自多个小麦及其祖先遗传背景的蜡质蛋白。三种二倍体祖先物种蜡质蛋白的推导氨基酸序列显示了几个关键的氨基酸替换，这可以解释在小麦蜡质蛋白电泳迁移率中观察到的差异。二倍体和多倍体小麦WX-A1蛋白表观分子量的微小差异是由氨基酸替换或预测蛋白质结构的变化所解释的。此外，分离并比较了代表各种二倍体、四倍体和六倍体小麦单个蜡质基因座的12个不同的部分基因组克隆。结果表明内含子4存在显著变异，并导致鉴定出小麦及其假定祖先的单个蜡质基因和基因组所特有的序列。观察到的序列变异在开发作为鉴定特定蜡质基因座和研究小麦各种蜡质突变体的分子标记方面具有很大潜力。
