Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T
Tohoku National Agricultural Experiment Station, Iwate, Japan.
Mol Gen Genet. 1995 Aug 21;248(3):253-9. doi: 10.1007/BF02191591.
The Waxy (Wx) protein has been identified as granule-bound starch synthase (GBSS; EC 24.1.21), which is involved in amylose synthesis in plants. Although common wheat (Triticum aestivum L.) has three Wx proteins, "partial waxy mutants" lacking one or two of the three proteins have been found. Using such partial waxy mutants, tetra- and hexaploid waxy mutants with endosperms that are stained red-brown by iodine were produced. Both mutants showed loss of Wx protein and amylose. This is the first demonstration of genetic modification of wheat starch.
蜡质（Wx）蛋白已被鉴定为颗粒结合型淀粉合酶（GBSS；EC 24.1.21），它参与植物直链淀粉的合成。虽然普通小麦（Triticum aestivum L.）有三种Wx蛋白，但已发现缺少这三种蛋白中的一种或两种的“部分蜡质突变体”。利用这些部分蜡质突变体，培育出了胚乳被碘染成红棕色的四倍体和六倍体蜡质突变体。这两种突变体均表现出Wx蛋白和直链淀粉的缺失。这是小麦淀粉基因改造的首次证明。
