McMahon K J, Renner E D, Allmaras G W, Anderson D K
Can J Microbiol. 1979 Jul;25(7):850-4. doi: 10.1139/m79-125.
A comparison was made of results obtained with a Brucella agar--gel immunodiffusion (AGID) test and the standard tube-agglutination test on 612 human sera. Agreement between the tests was 97% when the titer was 1:160 or higher. Of 448 sera that showed no agglutination titer, 447 were negative with the AGID test. Results of the AGID test were also compared to those obtained with the 2-mercaptoethanol (2-ME) agglutination test on 148 sera that demonstrated a standard tube-agglutination titer of 1:20 or higher. All sera with a 2-ME-agglutination titer of 1:40 or higher were positive with the AGID test. Of 123 sera that showed no 2-ME-agglutination titer, 21 were positive with the AGID test. Two of these 21 sera were obtained from patients with bacteriologically proven brucellosis, and eight were from abattoir employees with suspected but not bacteriologically proven brucellosis.
对612份人血清进行了布鲁氏菌琼脂凝胶免疫扩散（AGID）试验和标准试管凝集试验，并对结果进行了比较。当滴度为1:160或更高时，两种试验的一致性为97%。在448份未显示凝集滴度的血清中，447份AGID试验为阴性。还将AGID试验结果与用2-巯基乙醇（2-ME）凝集试验对148份标准试管凝集滴度为1:20或更高的血清所获得的结果进行了比较。所有2-ME凝集滴度为1:40或更高的血清AGID试验均为阳性。在123份未显示2-ME凝集滴度的血清中，21份AGID试验为阳性。这21份血清中有2份来自细菌学确诊为布鲁氏菌病的患者，8份来自怀疑患有但未经细菌学确诊的布鲁氏菌病的屠宰场员工。
