Mattila P, Astola J, Kumpulainen J
Agricultural Research Centre of Finland, Food Research, L-talo, 31600 Jokioinen, Finland.
J Agric Food Chem. 2000 Dec;48(12):5834-41. doi: 10.1021/jf000661f.
A high-performance liquid chromatographic (HPLC) method with in-line connected diode-array (DAD) and electro-array (EC) detection to identify and quantify 17 flavonoids in plant-derived foods is described. Catechins were extracted from the samples using ethyl acetate, and quantification of these compounds was performed with the EC detector. Other flavonoids were quantified with DAD after acid hydrolysis. The methods developed were effective for the determination of catechins and other flavonoids in plant-derived foods. Responses of the detection systems were linear within the range evaluated, 20-200 ng/injection (DAD) and 20-100 ng/injection (EC), with correlation coefficients exceeding 0.999. Coefficient of variation was under 10.5%, and recoveries of flavonoids ranged from 70 to 124%. Purity of the flavonoid peaks was confirmed by combining the spectral and voltammetric data.
描述了一种采用在线连接二极管阵列（DAD）和电化学阵列（EC）检测的高效液相色谱（HPLC）方法，用于鉴定和定量植物性食品中的17种黄酮类化合物。儿茶素采用乙酸乙酯从样品中提取，并用EC检测器对这些化合物进行定量。其他黄酮类化合物在酸水解后用DAD进行定量。所建立的方法对于测定植物性食品中的儿茶素和其他黄酮类化合物是有效的。检测系统的响应在评估范围内呈线性，即进样量为20 - 200 ng（DAD）和20 - 100 ng（EC）时，相关系数超过0.999。变异系数低于10.5%，黄酮类化合物的回收率在70%至124%之间。通过结合光谱和伏安数据确认了黄酮类化合物峰的纯度。
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