Nutrition and Food Science Department, XaRTA, INSA, Pharmacy School, University of Barcelona, Barcelona, Spain.
J Agric Food Chem. 2011 Jun 22;59(12):6353-9. doi: 10.1021/jf200625z. Epub 2011 Jun 1.
Flavonoids are a widely distributed group of polyphenolic compounds present in an extensive range of edible plants, notably Citrus species. This article reports a rapid, optimized, and validated method for the separation and quantification of flavonoids in three Citrus fruit extracts by ultrahigh performance liquid chromatography (UHPLC) using a photodiode array detector. This new procedure allowed the simultaneous separation and quantification of 11 selected flavonoids in 5.5 min, 8.2 times faster than that by HPLC analysis. The solvent consumption for each individual analysis was also reduced almost 6.2-fold. The most abundant component in the analyzed samples was naringin (299.06-544.36 mg 100 g⁻¹), followed by rutin (116.60-256.33 mg 100 g⁻¹) and quercetin (7.78-251.49 mg 100 g⁻¹). Isoquercitrin was found in a lower proportion (60.05-81.88 mg 100 g⁻¹). The method was completely validated, providing a sensitive analysis for flavonoid detection and showing satisfactory data for all the parameters tested. This methodology is cheaper, more environmentally friendly, and easier to perform than others previously described.
类黄酮是广泛分布的多酚化合物群，存在于大量可食用植物中，特别是柑橘属植物。本文报道了一种快速、优化和验证的方法，用于通过超高效液相色谱（UHPLC）使用光电二极管阵列检测器分离和定量三种柑橘属水果提取物中的类黄酮。该新工艺允许在 5.5 分钟内同时分离和定量 11 种选定的类黄酮，比 HPLC 分析快 8.2 倍。每个单独分析的溶剂消耗也减少了近 6.2 倍。在分析样品中最丰富的成分是柚皮苷（299.06-544.36mg 100g⁻¹），其次是芦丁（116.60-256.33mg 100g⁻¹）和槲皮素（7.78-251.49mg 100g⁻¹）。异槲皮苷的比例较低（60.05-81.88mg 100g⁻¹）。该方法经过全面验证，提供了一种用于类黄酮检测的灵敏分析方法，并对所有测试参数均显示出令人满意的数据。该方法比以前描述的方法更便宜、更环保、更易于执行。
