Anderson R A, Reddy J M, Oswald C, Zaneveld L J
Clin Chem. 1979 Oct;25(10):1780-2.
We describe a spectrophotometric assay for fructose in seminal plasma. The method is based on reduction of fructose by a commercially available preparation of sorbitol dehydrogenase (EC 1.1.1.14), with the concomitant oxidation of NADH. The initial rate of NADH oxidation, which is proportional to the fructose content of seminal plasma, can be measured either with a recording spectrophotometer or by conventional two-point kinetic assay. The method was as accurate, precise, and sensitive as, and more specific and rapid than, currently used colorimetric (resorcinol) methods for fructose determination. Values (mmol/L) for fructose in seminal plasma from several species are: man, 9 +/- 2 (SD); cynamolgus monkey (Macaca fasicicularis)., 108 +/- 19; bull, 30 +/- 1; and rabbit, 13 +/- 4. These values agree with previously published results. We believe the method is appropriate for both research and clinical use.
我们描述了一种用于测定精浆中果糖的分光光度法。该方法基于市售的山梨醇脱氢酶制剂（EC 1.1.1.14）对果糖的还原作用，同时伴有NADH的氧化。NADH氧化的初始速率与精浆中的果糖含量成正比，可使用记录分光光度计或通过传统的两点动力学分析法进行测量。该方法与目前用于测定果糖的比色法（间苯二酚法）一样准确、精密和灵敏，且更具特异性和快速性。几种物种精浆中果糖的含量（mmol/L）分别为：人类，9±2（标准差）；食蟹猴（猕猴），108±19；公牛，30±1；兔子，13±4。这些数值与先前发表的结果一致。我们认为该方法适用于研究和临床应用。
