Tuan R, Bortolato P C
Laboratório de Malacologia, Superintendência de Controle de Endemias, São Paulo, SP, 01027-000, Brasil.
Mem Inst Oswaldo Cruz. 2001 Apr;96(3):435-6. doi: 10.1590/s0074-02762001000300026.
Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.
嗜气管双脐螺是曼氏血吸虫吸虫的中间宿主之一，是一种雌雄同体的蜗牛物种。为了分析这些种群的遗传结构，我们采用了双严格PCR技术，在单一反应中用微卫星和任意引物获得遗传标记。
