Boos A
Institute of Anatomy, Department of Histology and Embryology, School of Veterinary Medicine Hannover, Germany.
Ital J Anat Embryol. 1998;103(4 Suppl 1):203-11.
Luteal tissue specimens of corpora lutea of oestrous cycle and pregnancy, post partum corpora lutea, and of luteinised follicular cysts of cows were harvested at slaughter. Cellular proliferation was examined employing Ki-67 immunocytochemistry. Ki-67 immunoreactivities could mainly be demonstrated within the nuclei of the theca interna and theca externa derived cell populations of cyclic corpora lutea. These cells obviously differentiated into vascular cells, connective tissue cells and small luteal cells. Proliferative activity was strongest in young corpora lutea and markedly reduced in the mature ones. During involution of corpora lutea of oestrous cycle Ki-67-positive cells were mainly found in vascular walls and connective tissue districts. Luteinised follicular cysts closely resembled cyclic corpora lutea. Corpora lutea of pregnancy exhibited foci of immunopositive cells close to blood vessels and connective tissue septae during whole gestation. Post partal luteal involution was characterised by Ki-67-positive cells within vascular walls and the surrounding stroma. Results suggest that luteal cellular proliferation is restricted mainly to theca interna-derived cells, which may differentiate to stromal cells and parenchymal cells.
在屠宰时采集处于发情周期和妊娠期的黄体、产后黄体以及母牛的黄素化卵泡囊肿的黄体组织样本。采用Ki-67免疫细胞化学方法检测细胞增殖情况。Ki-67免疫反应主要出现在周期性黄体的内膜和外膜来源细胞群体的细胞核内。这些细胞明显分化为血管细胞、结缔组织细胞和小黄体细胞。增殖活性在年轻黄体中最强，在成熟黄体中明显降低。在发情周期黄体退化过程中，Ki-67阳性细胞主要见于血管壁和结缔组织区域。黄素化卵泡囊肿与周期性黄体非常相似。妊娠黄体在整个妊娠期均表现出靠近血管和结缔组织间隔的免疫阳性细胞灶。产后黄体退化的特征是血管壁和周围基质中有Ki-67阳性细胞。结果表明，黄体细胞增殖主要局限于内膜来源的细胞，这些细胞可能分化为基质细胞和实质细胞。
