Khan-Dawood F S
Am J Anat. 1987 May;179(1):18-24. doi: 10.1002/aja.1001790104.
Corpora lutea, corpora albicantia, and ovarian stroma from normal human premenopausal ovaries were examined for the presence of oxytocin and neurophysin by using highly specific antisera and peroxidase-antiperoxidase light-microscopic immunohistochemistry. Oxytocin and neurophysin immunoreactivity was found in some but not all cells of the corpora lutea obtained on days 19 to 24 of the menstrual cycle. Stromal tissue and corpora albicantia did not give a positive reaction for either of these peptides, and negative results were also obtained with corpora lutea of mid- and term-pregnancy and preovulatory follicles. Specificity of the immunohistochemical reaction was confirmed by immunoabsorption tests. The specific localization of immunoreactive oxytocin and neurophysin in corpora lutea of the human menstrual cycle directly demonstrates the presence of oxytocin- and neurophysin-positive cells within the human corpus luteum.
利用高特异性抗血清和过氧化物酶-抗过氧化物酶光镜免疫组织化学方法，检测正常人类绝经前卵巢中的黄体、白体和卵巢基质中是否存在催产素和神经垂体素。在月经周期第19至24天获得的黄体的部分而非全部细胞中发现了催产素和神经垂体素免疫反应性。基质组织和白体对这两种肽均未产生阳性反应，妊娠中期和足月的黄体以及排卵前卵泡也得到了阴性结果。免疫吸收试验证实了免疫组织化学反应的特异性。人类月经周期黄体中免疫反应性催产素和神经垂体素的特异性定位直接证明了人类黄体中存在催产素和神经垂体素阳性细胞。
