Sato G, Desmond W, Kelly F
Cancer Chemother Rep. 1975 Jan-Feb;59(1):47-9.
A nude mouse colony has been established with a capacity of over 1000 mice. These mice have been injected with human prostatic tumors. A spleen injection method has been developed which enables us to follow the growth of tumor cells in the animal for short periods of time and to assess the effects of hormones on this growth. We have also studied the behavior of several animal cell lines in culture and in nude mice as possible models for a hormone-dependent human prostatic tumor. By using nude mice, we have been able to show that apparent "normal" revertants of cancer cells are actually antigenic variants which can be used to immunize animals against the original tumor cells. A melanoma cell line has been developed whose growth appears to be markedly enhanced by androgens. Rat ovarian cell lines have been developed whose growth and viability are hormone dependent in vivo and in vitro.
已建立了一个可容纳1000多只小鼠的裸鼠种群。这些小鼠已被注射了人类前列腺肿瘤。已开发出一种脾脏注射方法，使我们能够在短时间内跟踪动物体内肿瘤细胞的生长情况，并评估激素对这种生长的影响。我们还研究了几种动物细胞系在培养物中和裸鼠体内作为激素依赖性人类前列腺肿瘤可能模型的行为。通过使用裸鼠，我们已经能够证明癌细胞明显的“正常”回复突变体实际上是抗原变体，可用于免疫动物以对抗原始肿瘤细胞。已开发出一种黑色素瘤细胞系，其生长似乎因雄激素而显著增强。已开发出大鼠卵巢细胞系，其生长和活力在体内和体外均依赖激素。
