Bailey M J, Gazet J C, Peckham M J
Br J Cancer. 1980 Oct;42(4):524-9. doi: 10.1038/bjc.1980.275.
Eight serially transplantable human breast-cancer xenograft lines have been established in immune-suppressed mice. Specimens from 102 primary and secondary lesions obtained at surgery from 80 patients were implanted into mice immune-suppressed by thymectomy and whole-body irradiation. A number of variations of implantation site, transplantation technique, method of immune suppression and hormonal manipulation of the host were tried in an attempt to increase the take rate, but without success. The 8 lines established have been serially transplanted into further immune-suppressed mice for at least 2 passages, and appear to maintain characteristic human histopathology, chromosome number and tumour-marker production. None of the tumours show hormone sensitivity. The poor take rate may be a reflection of the biological nature of breast cancer rather than a failure of the immune-deprivation technique, as many other human tumours grow well as xenografts in this system.
在免疫抑制小鼠中已建立了8个可连续传代移植的人乳腺癌异种移植系。从80例患者手术获取的102个原发性和继发性病变标本被植入经胸腺切除和全身照射免疫抑制的小鼠体内。尝试了多种植入部位、移植技术、免疫抑制方法和宿主激素处理的变化，以提高成功率，但未成功。已建立的8个系已连续移植到其他免疫抑制小鼠中至少传代2次，并且似乎保持了典型的人类组织病理学、染色体数目和肿瘤标志物产生。所有肿瘤均未表现出激素敏感性。成功率低可能反映了乳腺癌的生物学特性，而非免疫剥夺技术的失败，因为许多其他人类肿瘤在该系统中作为异种移植生长良好。
