Effects of cis-diamminedichloroplatinum (NSC 119875) on murine and human hemopoietic precursor cells.

The effects of cis-diamminedichloroplatinum (NSC 119875) on murine and human hemopoietic precursor cells were studied in culture. When murine cells from normal and actively regenerating marrows were exposed to cis-diamminedichloroplatinum in culture, washed free of the agent, and assayed for the surviving hemopoietic precursor cells, similar sensitivity curves were obtained. This result indicates the absence of cell-cycle dependency of cis-diaminedichloroplatinum. When marrow cells were exposed to cis-diamminedichloroplatinum in culture at various temperatures, only minimal reduction of cytotoxicity was noted at 4 degrees. This observation, unlike that from the experiment with nitrogen mustard, suggests that the transport of this agent is by passive diffusion. Finally, when the sensitivity of human hemopoietic precursor cells to this agent was assessed using similar conditions and compared to that of murine hemopoietic precursor cells, a significant species difference was observed.