Singh S N, Vats P, Kumria M M, Ranganathan S, Shyam R, Arora M P, Jain C L, Sridharan K
Nutrition Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi-110054, India.
Eur J Appl Physiol. 2001 Mar;84(3):233-7. doi: 10.1007/s004210170010.
Reduced and oxidised glutathione (GSH and GSSG) contents, and glutathione reductase, and glutathione S-transferase activities were studied in the livers, muscles, and blood/erythrocytes of male Sprague-Dawley rats exposed to intermittent hypoxia (6 h.day-1) at a simulated altitude of 7,620 m for 1, 7, 14, and 21 days. Significant decreases in GSH and increases in GSSG contents were observed in the muscles and blood of hypoxia-exposed rats in comparison to unexposed rats. Significant declines in GSH content by 43% and 45% respectively in muscles and blood were observed in the group exposed for 1 day which tended to recover on subsequent exposure. Glutathione reductase and glutathione S-transferase activities were decreased in the livers and erythrocytes of hypoxia-exposed rats, but were increased significantly in muscle. Lipid peroxidation was also increased in the livers and muscles of exposed rats. The changes were indicative of an increased production of reactive oxygen species and an impairment of drug and xenobiotic metabolism during exposure to high altitude hypoxia.
研究了暴露于间歇性低氧（每天6小时）、模拟海拔7620米环境下1天、7天、14天和21天的雄性斯普拉格-道利大鼠肝脏、肌肉以及血液/红细胞中的还原型和氧化型谷胱甘肽（GSH和GSSG）含量、谷胱甘肽还原酶以及谷胱甘肽S-转移酶活性。与未暴露大鼠相比，暴露于低氧环境的大鼠肌肉和血液中GSH含量显著降低，GSSG含量升高。暴露1天的组中，肌肉和血液中的GSH含量分别显著下降43%和45%，后续暴露时趋于恢复。暴露于低氧环境的大鼠肝脏和红细胞中谷胱甘肽还原酶和谷胱甘肽S-转移酶活性降低，但肌肉中显著升高。暴露大鼠的肝脏和肌肉中脂质过氧化也增加。这些变化表明在暴露于高海拔低氧环境期间活性氧生成增加以及药物和外源性物质代谢受损。
