Graves L M, Swaminathan B
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Int J Food Microbiol. 2001 Apr 11;65(1-2):55-62. doi: 10.1016/s0168-1605(00)00501-8.
PulseNet is a national network of pubic health and food regulatory laboratories established in the US to detect clusters of foodborne disease and respond quickly to foodborne outbreak investigations. PulseNet laboratories currently subtype Escherichia coli O157:H7, non-typhoidal Salmonella, and Shigella isolates by a highly standardized 1-day pulsed-field gel electrophoresis (PFGE), and exchange normalized DNA "fingerprint" patterns via the Internet. We describe a standardized molecular subtyping protocol for subtyping Listeria monocytogenes that was recently added to PulseNet. The subtyping can be completed within 30 h from the time a pure culture of the bacteria is obtained.
脉冲网络是美国建立的一个由公共卫生和食品监管实验室组成的全国性网络，用于检测食源性疾病集群，并对食源性疾病暴发调查迅速做出反应。脉冲网络实验室目前通过高度标准化的1天脉冲场凝胶电泳（PFGE）对大肠杆菌O157:H7、非伤寒沙门氏菌和志贺氏菌分离株进行亚型分析，并通过互联网交换标准化的DNA“指纹”图谱。我们描述了一种用于单核细胞增生李斯特菌亚型分析的标准化分子分型方案，该方案最近已被添加到脉冲网络中。从获得细菌纯培养物之时起，30小时内即可完成亚型分析。
