Martin Paul, Jacquet Christine, Goulet Véronique, Vaillant Véronique, De Valk Henriette
Laboratoire des Listeria, Centre National de Référence des Listeria, Institut Pasteur, Paris, France.
Foodborne Pathog Dis. 2006 Fall;3(3):303-8. doi: 10.1089/fpd.2006.3.303.
A feasibility study on standardized subtyping of Listeria monocytogenes by pulsed-field gel electrophoresis (PFGE) was performed in 2003 in Europe. The aim of the project was to identify veterinary, food, and public health reference laboratories that were willing to participate in the molecular surveillance of Listeria infections in Europe, as is done with PulseNet USA, and to test if the participants could generate results that were comparable with each other. A panel of strains and the methodology were sent to European laboratories, and images of PFGE gels were transferred electronically, then analyzed visually and with image analysis software. Only nine out of 43 gels were not fully satisfactory at the visual evaluation step stage. Computerized analysis showed that only three out of 33 gels gave dendrograms totally different from the reference dendrograms. Thus, the objectives of the study were met: most human and food European laboratories concerned with typing L. monocytogenes participated, images were transmitted electronically, and the overall quality of the gels was good.
2003年在欧洲开展了一项关于采用脉冲场凝胶电泳（PFGE）对单核细胞增生李斯特菌进行标准化分型的可行性研究。该项目的目的是确定愿意参与欧洲李斯特菌感染分子监测的兽医、食品和公共卫生参考实验室，就如同美国脉冲网络所开展的工作那样，并测试参与者能否得出相互可比的结果。一组菌株和方法被发送到欧洲各实验室，PFGE凝胶图像通过电子方式传输，然后进行目视分析并使用图像分析软件进行分析。在目视评估阶段，43份凝胶中只有9份不太令人满意。计算机分析表明，33份凝胶中只有3份给出的树形图与参考树形图完全不同。因此，该研究的目标得以实现：大多数涉及单核细胞增生李斯特菌分型的欧洲人类和食品实验室参与其中，图像通过电子方式传输，并且凝胶的整体质量良好。
