Song L, Zmijewski J W, Jope R S
Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, 35294-0017, USA.
Biochem Biophys Res Commun. 2001 Apr 27;283(1):102-6. doi: 10.1006/bbrc.2001.4742.
RGS2, a Regulators of G-protein Signaling family member, regulates signaling activities of G-proteins, and RGS2 itself is controlled in part by regulation of its expression. This investigation extended previous studies of the regulation of RGS2 expression by examining the effects of stress, differentiation, and signaling activities on RGS2 mRNA level in human neuroblastoma SH-SY5Y cells. Cell stress induced by heat shock rapidly and transiently increased RGS2 mRNA levels, whereas differentiation to a neuronal phenotype reduced basal RGS2 mRNA levels by 50%. RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Thus, RGS2 expression is regulated by stress and differentiation, as well as by second messenger signaling, and transfected GFP-RGS2 is predominantly nuclear.
RGS2是G蛋白信号调节家族的成员之一，它调节G蛋白的信号活动，并且RGS2自身部分受其表达调控的影响。本研究通过检测应激、分化及信号活动对人神经母细胞瘤SH-SY5Y细胞中RGS2 mRNA水平的影响，扩展了之前对RGS2表达调控的研究。热休克诱导的细胞应激迅速且短暂地增加了RGS2 mRNA水平，而向神经元表型的分化使基础RGS2 mRNA水平降低了50%。热休克、卡巴胆碱及蛋白激酶C的激活使分化细胞中的RGS2 mRNA水平升高。在瞬时转染绿色荧光蛋白标记的RGS2后，共聚焦显微镜观察到其主要定位于细胞核。因此，RGS2的表达受应激、分化以及第二信使信号的调控，并且转染的绿色荧光蛋白-RGS2主要定位于细胞核。
