Zmijewski J W, Song L, Harkins L, Cobbs C S, Jope R S
Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama, 35294-0017, USA.
Arch Biochem Biophys. 2001 Aug 15;392(2):192-6. doi: 10.1006/abbi.2001.2430.
RGS2, a regulators of G-protein signaling family member, regulates G-protein signaling and is itself controlled in part by regulated expression. We tested if cell stress regulates RGS2 expression in human astrocytoma 1321N1 cells. Treatment with H2O2 increased RGS2 mRNA levels time- and concentration-dependently, with 200 microM H2O2 causing an approximately eightfold increase after 2 h. Peroxynitrite and heat shock also increased RGS2 mRNA levels. H2O2-induced RGS2 expression was negatively regulated by phosphoinositide-3-kinase and extracellular signal-regulated kinases. H2O2 also concentration-dependently increased RGS2 protein levels, and the RGS2 appeared to be predominantly in the nucleus. These results demonstrate that RGS2 expression is up-regulated by cell stress.
RGS2是G蛋白信号调节家族的成员之一，它调节G蛋白信号传导，其自身部分受表达调控的控制。我们测试了细胞应激是否调节人星形细胞瘤1321N1细胞中RGS2的表达。用H2O2处理可使RGS2 mRNA水平呈时间和浓度依赖性增加，200 microM H2O2在2小时后可使RGS2 mRNA水平增加约八倍。过氧亚硝酸盐和热休克也可增加RGS2 mRNA水平。H2O2诱导的RGS2表达受磷酸肌醇-3-激酶和细胞外信号调节激酶的负调控。H2O2还可使RGS2蛋白水平呈浓度依赖性增加，且RGS2似乎主要位于细胞核中。这些结果表明，细胞应激可上调RGS2的表达。
