Kubota T, Yokosawa N, Yokota S, Fujii N
Department of Microbiology, Sapporo Medical University School of Medicine, South 1, West 16, Chou-ku, Sapporo, Hokkaido, 060-8556, Japan.
Biochem Biophys Res Commun. 2001 Apr 27;283(1):255-9. doi: 10.1006/bbrc.2001.4764.
It has been reported that interferon (IFN)-alpha/gamma signal transduction pathway is blocked in several cell lines persistently infected with mumps virus (MV) through decrease of STAT-1alpha. Expression of the MV structural V protein (MV-V) or C terminal CYS-RICH region of the V protein (MV-Vsp) inhibited the establishment of the antivirus state induced by IFN, but not by expression of the MV-P protein. Suppression of IFN-induced STAT-1alpha, STAT-2, and IRF-9 (p48) induction was also recognized in the cells transfected with expression vector of the MV-V (pTM-V) or MV-Vsp (pTM-Vsp) protein, even though it was in the absence of the other virus protein. It is supposed that the cysteine-rich domain of V protein (Vsp) is involved in the suppression of the IFN signal transduction pathway.
据报道，在几种持续感染腮腺炎病毒（MV）的细胞系中，由于STAT-1α的减少，干扰素（IFN）-α/γ信号转导途径被阻断。MV结构V蛋白（MV-V）或V蛋白的C末端富含半胱氨酸区域（MV-Vsp）的表达抑制了IFN诱导的抗病毒状态的建立，但MV-P蛋白的表达则没有这种作用。在用MV-V（pTM-V）或MV-Vsp（pTM-Vsp）蛋白表达载体转染的细胞中，也观察到IFN诱导的STAT-1α、STAT-2和IRF-9（p48）诱导的抑制，即使不存在其他病毒蛋白。推测V蛋白（Vsp）的富含半胱氨酸结构域参与了IFN信号转导途径的抑制。
