Collett G P, Robson C N, Mathers J C, Campbell F C
Department of Surgery, The Cancer Centre, Queen's University Belfast.
Carcinogenesis. 2001 May;22(5):821-5. doi: 10.1093/carcin/22.5.821.
Curcumin, the active ingredient of the rhizome of Curcuma longa, promotes apoptosis and may have chemopreventive properties. This study investigates the effects of curcumin on apoptosis and tumorigenesis in male Apc(min) mice treated with the human dietary carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Intestinal epithelial apoptotic index in response to PhIP treatment was approximately twice as great in the wild-type C57BL/6 APC(+/+) strain than in Apc(min) mice (3.7% Apc(+/+) versus 1.9% Apc(min); P < 0.001). PhIP promoted tumour formation in Apc(min) proximal small intestine (4.6 tumours per mouse, PhIP treated versus 2.1 tumours per mouse, control untreated; P < 0.05). Curcumin enhanced PhIP-induced apoptosis (4.0% curcumin + PhIP versus 2.1% PhIP alone; P < 0.01) and inhibited PhIP-induced tumorigenesis in the proximal small intestine of Apc(min) mice (2.2 tumours per mouse, curcumin + PhIP versus 4.6 tumours per mouse PhIP alone; P < 0.05). This study shows that the Apc(min) genotype is associated with resistance to PhIP-induced apoptosis in intestinal epithelium. Curcumin attenuates Apc(min) resistance to PhIP-induced apoptosis and inhibits PhIP-induced tumorigenesis in proximal Apc(min) mouse small intestine.
姜黄素是姜黄根茎的活性成分，可促进细胞凋亡，可能具有化学预防特性。本研究调查了姜黄素对用人类饮食致癌物2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）处理的雄性Apc（min）小鼠细胞凋亡和肿瘤发生的影响。野生型C57BL/6 APC（+/+）品系对PhIP处理的肠道上皮细胞凋亡指数约为Apc（min）小鼠的两倍（Apc（+/+）为3.7%，Apc（min）为1.9%；P < 0.001）。PhIP促进了Apc（min）小鼠近端小肠肿瘤的形成（PhIP处理组每只小鼠有4.6个肿瘤，未处理的对照组每只小鼠有2.1个肿瘤；P < 0.05）。姜黄素增强了PhIP诱导的细胞凋亡（姜黄素+PhIP组为4.0%，单独使用PhIP组为2.1%；P < 0.01），并抑制了PhIP诱导的Apc（min）小鼠近端小肠肿瘤发生（姜黄素+PhIP组每只小鼠有2.2个肿瘤，单独使用PhIP组每只小鼠有4.6个肿瘤；P < 0.05）。本研究表明，Apc（min）基因型与肠道上皮对PhIP诱导的细胞凋亡的抗性有关。姜黄素减弱了Apc（min）对PhIP诱导的细胞凋亡的抗性，并抑制了PhIP诱导的Apc（min）小鼠近端小肠肿瘤发生。
