Leclerc D, Stavolone L, Meier E, Guerra-Peraza O, Herzog E, Hohn T
CHUQ, Pav.CHUL, Center de Recherche en Infectiologie, P. Quebec, Canada.
Virus Genes. 2001 Mar;22(2):159-65. doi: 10.1023/a:1008121228637.
Using the yeast two-hybrid system, we show that the ORF III product of cauliflower mosaic virus (pIII) interacts through its C-terminus with the viral coat protein. The last five amino acids of pIII were essential for the interaction and virus infectivity. Deletion of the last three amino acids or the mutation F129A decreased the strength of the interaction by 90%. We further show that pIII is closely associated with virus particles found in the inclusion bodies of infected plants but not in viral particles released from the inclusion bodies by urea treatment.
利用酵母双杂交系统，我们发现花椰菜花叶病毒（CaMV）的ORF III产物（pIII）通过其C末端与病毒外壳蛋白相互作用。pIII的最后五个氨基酸对于这种相互作用和病毒感染性至关重要。删除最后三个氨基酸或F129A突变会使相互作用强度降低90%。我们进一步表明，pIII与感染植物包涵体中的病毒粒子紧密相关，但与通过尿素处理从包涵体释放的病毒粒子无关。
