Tran Q L, Adnyana I K, Tezuka Y, Nagaoka T, Tran Q K, Kadota S
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
J Nat Prod. 2001 Apr;64(4):456-61. doi: 10.1021/np000393f.
The methanol extract of Vietnamese ginseng (Panax vietnamensis) was found to possess hepatocytoprotective effects on D-galactosamine (D-GalN)/tumor necrosis factor-alpha (TNF-alpha)-induced cell death in primary cultured mouse hepatocytes. Further chemical investigation of the extract afforded two new dammarane-type triterpene saponins, ginsenoside Rh(5) (1) and vina-ginsenoside R(25) (2), as well as eight known dammarane-type triterpene saponins, majonoside R(2) (3), pseudo-ginsenoside RT(4) (4), vina-ginsenosides R(1) (5), R(2) (6), and R(10) (7), ginsenosides Rg(1) (8), Rh(1) (9), and Rh(4) (10), and a known sapogenin protopanaxatriol oxide II (11). Their structures were elucidated on the basis of spectral analysis. In addition, by the using LC-electrospray ionization (ESI)-MS method, five known saponins, ginsenosides Rb(1), Rb(2), Rc, Rd, and Re (12--16), were also identified in the extract. Among the compounds isolated, majonoside R(2) (3), the main saponin in Vietnamese ginseng, showed strong protective activity against D-GalN/TNF-alpha-induced cell death in primary cultured mouse hepatocytes. This demonstrates that the hepatocytoprotective effect of Vietnamese ginseng is due to dammarane-type triterpene saponins that have an ocotillol-type side chain, a characteristic constituent of Vietnamese ginseng.
越南人参（Panax vietnamensis）的甲醇提取物被发现对原代培养的小鼠肝细胞中由D - 半乳糖胺（D - GalN）/肿瘤坏死因子 - α（TNF - α）诱导的细胞死亡具有肝细胞保护作用。对该提取物进行进一步的化学研究，得到了两种新的达玛烷型三萜皂苷，人参皂苷Rh(5)（1）和越南人参皂苷R(25)（2），以及八种已知的达玛烷型三萜皂苷，majonoside R(2)（3）、假人参皂苷RT(4)（4）、越南人参皂苷R(1)（5）、R(2)（6）、R(10)（7）、人参皂苷Rg(1)（8）、Rh(1)（9）和Rh(4)（10），还有一种已知的皂苷元原人参三醇氧化物II（11）。它们的结构通过光谱分析得以阐明。此外，通过液相色谱 - 电喷雾电离（ESI）-质谱法，提取物中还鉴定出了五种已知的皂苷，人参皂苷Rb(1)、Rb(2)、Rc、Rd和Re（12 - 16）。在分离得到的化合物中，越南人参中的主要皂苷majonoside R(2)（3）对原代培养的小鼠肝细胞中D - GalN/TNF - α诱导的细胞死亡表现出强烈的保护活性。这表明越南人参的肝细胞保护作用归因于具有奥科梯罗醇型侧链的达玛烷型三萜皂苷，这是越南人参的一种特征成分。
