Ito H, Sugiyama M, Masubuchi K, Mori Y, Minamoto N
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan.
Virus Res. 2001 Jun;75(2):123-38. doi: 10.1016/s0168-1702(01)00234-9.
The nucleotide sequences encoding four structural proteins (VP1-4) and six nonstructural proteins (NSP1-6) of avian rotavirus PO-13 were determined. Based on the results of earlier sequencing studies [Ito et al., 1995, Sequence analysis of cDNA for the VP6 protein of group A avian rota viruses. Arch. Vriol. 140, 605-612; Rohwedder et al., 1997, Chicken rotavirus Ch-1 shows a second type of avian VP6 gene, Virus Genes 15, 65-71; Rohwedder et al., 1997, Bovine rotavirus 993/83 shows a third subtype of avian VP7 protein, Virus Genes 14, 147-151], determination of PO-13 genome sequence has been completed. The PO-13 genome is 18845 nucleotides in length. It is 290 nucleotides longer than the genome of SA11. The amino acid sequence homology between PO-13 and mammalian rotaviruses ranged from 76-77% (VP1) to 16-18% (NSP1). The features of gene and amino acid sequence were compared with those of the corresponding protein of mammalian rotaviruses. Based on results of the phylogenetic analyses of NSP1, we speculate that an ancestral rotavirus could have separated into groups A, B and C rotaviruses at an early evolutionary stage and that group A rotavirus separated into mammalian and avian rotaviruses with host evolution.
测定了禽轮状病毒PO-13编码四种结构蛋白（VP1-4）和六种非结构蛋白（NSP1-6）的核苷酸序列。基于早期测序研究的结果[伊藤等人，1995年，A组禽轮状病毒VP6蛋白的cDNA序列分析。《病毒学文献》140，605 - 612；罗韦德德等人，1997年，鸡轮状病毒Ch-1显示出第二种禽VP6基因类型，《病毒基因》15，65 - 71；罗韦德德等人，1997年，牛轮状病毒993/83显示出禽VP7蛋白的第三种亚型，《病毒基因》14，147 - 151]，已完成PO-13基因组序列的测定。PO-13基因组长度为18845个核苷酸。它比SA11的基因组长290个核苷酸。PO-13与哺乳动物轮状病毒之间的氨基酸序列同源性范围从76 - 77%（VP1）到16 - 18%（NSP1）。将基因和氨基酸序列的特征与哺乳动物轮状病毒相应蛋白的特征进行了比较。基于NSP1系统发育分析的结果，我们推测一种祖先轮状病毒可能在进化早期就分化为A、B和C组轮状病毒，并且A组轮状病毒随着宿主进化分化为哺乳动物和禽轮状病毒。
