Mori Yoshio, Borgan Mohammed Ali, Ito Naoto, Sugiyama Makoto, Minamoto Nobuyuki
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Virus Res. 2002 Oct;89(1):145-51. doi: 10.1016/s0168-1702(02)00112-0.
We determined the NSP4 sequences of turkey rotavirus strains Ty-1 and Ty-3 and a chicken rotavirus, strain Ch-1, and compared these sequences with those of a pigeon rotavirus, strain PO-13, and mammalian rotaviruses. The turkey strains and PO-13 were found to be closely related (90-97% homologies). Ch-1 NSP4 was distinctly different from other avian rotavirus NSP4s, with 78-79% homologies. The NSP4 sequences of avian rotaviruses were found to be 6-7 amino acids shorter than those of all mammalian strains and to have considerably low identities (31-37%) with them. Therefore, it seems highly likely that the NSP4 genes of avian rotaviruses are classified into two NSP4 genotypes distinct from those of mammalian rotaviruses. The enterotoxin domain in NSP4 is conserved in terms of its sequential and structural properties despite extremely low homologies in the full lengths of NSP4s in avian and mammalian rotaviruses.
我们测定了火鸡轮状病毒毒株Ty-1和Ty-3以及鸡轮状病毒毒株Ch-1的NSP4序列，并将这些序列与鸽轮状病毒毒株PO-13和哺乳动物轮状病毒的序列进行了比较。发现火鸡毒株与PO-13密切相关（同源性为90-97%）。Ch-1 NSP4与其他禽轮状病毒NSP4明显不同，同源性为78-79%。发现禽轮状病毒的NSP4序列比所有哺乳动物毒株的序列短6-7个氨基酸，并且与它们的同源性相当低（31-37%）。因此，禽轮状病毒的NSP4基因似乎很有可能被分为与哺乳动物轮状病毒不同的两种NSP4基因型。尽管禽和哺乳动物轮状病毒NSP4全长的同源性极低，但NSP4中的肠毒素结构域在其序列和结构特性方面是保守的。
