• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

磷酸泛酰巯基乙胺基转移酶同源物对于发光杆菌支持昆虫病原线虫嗜菌异小杆线虫的生长和繁殖至关重要。

A Phosphopantetheinyl transferase homolog is essential for Photorhabdus luminescens to support growth and reproduction of the entomopathogenic nematode Heterorhabditis bacteriophora.

作者信息

Ciche T A, Bintrim S B, Horswill A R, Ensign J C

机构信息

Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.

出版信息

J Bacteriol. 2001 May;183(10):3117-26. doi: 10.1128/JB.183.10.3117-3126.2001.

DOI:10.1128/JB.183.10.3117-3126.2001
PMID:11325940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC95212/
Abstract

The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the absence of viable P. luminescens cells. In an effort to identify bacterial factors that are required for nematode growth and reproduction, transposon-induced mutants of P. luminescens were screened for the loss of the ability to support growth and reproduction of H. bacteriophora nematodes. One mutant, NGR209, consistently failed to support nematode growth and reproduction. This mutant was also defective in the production of siderophore and antibiotic activities. The transposon was inserted into an open reading frame homologous to Escherichia coli EntD, a 4'-phosphopantetheinyl (Ppant) transferase, which is required for the biosynthesis of the catechol siderophore enterobactin. Ppant transferases catalyze the transfer of the Ppant moiety from coenzyme A to a holo-acyl, -aryl, or -peptidyl carrier protein(s) required for the biosynthesis of fatty acids, polyketides, or nonribosomal peptides. Possible roles of a Ppant transferase in the ability of P. luminescens to support nematode growth and reproduction are discussed.

摘要

发光光杆状菌是昆虫病原线虫嗜菌异小杆线虫的一种共生菌。线虫感染昆虫幼虫需要这种细菌,并且将其作为生长和繁殖的底物。在没有活的发光光杆状菌细胞的情况下,线虫无法在昆虫宿主体内或人工培养基上生长和繁殖。为了确定线虫生长和繁殖所需的细菌因子,对发光光杆状菌的转座子诱导突变体进行了筛选,以寻找支持嗜菌异小杆线虫生长和繁殖能力丧失的突变体。一个突变体NGR209始终无法支持线虫的生长和繁殖。该突变体在铁载体的产生和抗生素活性方面也存在缺陷。转座子插入到一个与大肠杆菌EntD同源的开放阅读框中,EntD是一种4'-磷酸泛酰巯基乙胺(Ppant)转移酶,是儿茶酚铁载体肠杆菌素生物合成所必需的。Ppant转移酶催化Ppant部分从辅酶A转移到脂肪酸、聚酮化合物或非核糖体肽生物合成所需的全酰基、芳基或肽基载体蛋白上。本文讨论了Ppant转移酶在发光光杆状菌支持线虫生长和繁殖能力方面的可能作用。

相似文献

1
A Phosphopantetheinyl transferase homolog is essential for Photorhabdus luminescens to support growth and reproduction of the entomopathogenic nematode Heterorhabditis bacteriophora.磷酸泛酰巯基乙胺基转移酶同源物对于发光杆菌支持昆虫病原线虫嗜菌异小杆线虫的生长和繁殖至关重要。
J Bacteriol. 2001 May;183(10):3117-26. doi: 10.1128/JB.183.10.3117-3126.2001.
2
Photobactin: a catechol siderophore produced by Photorhabdus luminescens, an entomopathogen mutually associated with Heterorhabditis bacteriophora NC1 nematodes.光杆菌素:一种由发光光杆状菌产生的儿茶酚型铁载体,发光光杆状菌是一种与嗜线虫致病杆菌NC1线虫相互关联的昆虫病原菌。
Appl Environ Microbiol. 2003 Aug;69(8):4706-13. doi: 10.1128/AEM.69.8.4706-4713.2003.
3
Effect of Photorhabdus luminescens phase variants on the in vivo and in vitro development and reproduction of the entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae.发光杆菌相变体对昆虫病原线虫嗜菌异小杆线虫和小卷蛾斯氏线虫体内外发育及繁殖的影响
FEMS Microbiol Ecol. 2001 May;35(3):239-247. doi: 10.1111/j.1574-6941.2001.tb00809.x.
4
HETERORHABDITIS BACTERIOPHORA NEMATODES ARE SENSITIVE TO THE BACTERIAL PATHOGEN PHOTORHABDUS ASYMBIOTICA.嗜线虫致病杆菌能感染异小杆线虫。
J Parasitol. 2023 Jan 1;109(1):11-14. doi: 10.1645/22-55.
5
Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts.在与无脊椎动物宿主相互作用过程中 Photorhabdus Dam 甲基转移酶的作用。
PLoS One. 2019 Oct 9;14(10):e0212655. doi: 10.1371/journal.pone.0212655. eCollection 2019.
6
Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis.嗜线虫致病杆菌(Photorhabdus temperata)基因组解析及转座子突变体文库构建以鉴定在致病过程中发生改变的运动突变体
J Bacteriol. 2015 Jul;197(13):2201-2216. doi: 10.1128/JB.00197-15. Epub 2015 Apr 27.
7
Involvement of Vitamin B6 Biosynthesis Pathways in the Insecticidal Activity of Photorhabdus luminescens.维生素B6生物合成途径参与发光杆菌的杀虫活性
Appl Environ Microbiol. 2016 May 31;82(12):3546-3553. doi: 10.1128/AEM.00522-16. Print 2016 Jun 15.
8
HdfR is a regulator in Photorhabdus luminescens that modulates metabolism and symbiosis with the nematode Heterorhabditis.HdfR 是发光杆菌中的一种调节因子,可调节代谢并与线虫异小杆线虫共生。
Environ Microbiol. 2012 Apr;14(4):953-66. doi: 10.1111/j.1462-2920.2011.02669.x. Epub 2011 Dec 12.
9
Photorhabdus luminescens LN2 requires rpoS for nematicidal activity and nematode development.发光光杆状菌LN2的杀线虫活性和线虫发育需要rpoS。
FEMS Microbiol Lett. 2016 Mar;363(6). doi: 10.1093/femsle/fnw035. Epub 2016 Feb 15.
10
Genetic and proteomic characterization of rpoB mutations and their effect on nematicidal activity in Photorhabdus luminescens LN2.rpoB 基因突变的遗传和蛋白质组学特征及其对发光杆菌 LN2 杀线虫活性的影响。
PLoS One. 2012;7(8):e43114. doi: 10.1371/journal.pone.0043114. Epub 2012 Aug 17.

引用本文的文献

1
The insight into the biology of five homologous lectins produced by the entomopathogenic bacterium and nematode symbiont Photorhabdus laumondii.对昆虫病原细菌和线虫共生体发光光杆状菌产生的五种同源凝集素生物学特性的深入了解。
Glycobiology. 2025 Jun 2;35(7). doi: 10.1093/glycob/cwaf033.
2
Pheno- and genotyping in vitro dauer juvenile recovery in the nematode Heterorhabditis bacteriophora.在线虫嗜菌异小杆线虫中进行表型和基因型鉴定,以恢复 dauer 幼体。
Appl Microbiol Biotechnol. 2023 Dec;107(23):7181-7196. doi: 10.1007/s00253-023-12775-y. Epub 2023 Sep 21.
3
and Symbiosis: A Natural Mine of Bioactive Compounds.以及《共生:生物活性化合物的天然宝库》
Front Microbiol. 2022 Mar 29;13:790339. doi: 10.3389/fmicb.2022.790339. eCollection 2022.
4
Molecular Regulators of Entomopathogenic Nematode-Bacterial Symbiosis.昆虫病原线虫-细菌共生的分子调控因子。
Results Probl Cell Differ. 2020;69:453-468. doi: 10.1007/978-3-030-51849-3_17.
5
Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed β-Propeller Lectin Family.凝集素 PLL3,七叶β-螺旋桨结构家族的新型单体成员。
Molecules. 2019 Dec 11;24(24):4540. doi: 10.3390/molecules24244540.
6
Improving the annotation of the Heterorhabditis bacteriophora genome.改进异小杆线虫基因组的注释。
Gigascience. 2018 Apr 1;7(4). doi: 10.1093/gigascience/giy034.
7
Genome sequence and comparative analysis of a putative entomopathogenic Serratia isolated from Caenorhabditis briggsae.从秀丽隐杆线虫中分离出的一种假定昆虫病原性沙雷氏菌的基因组序列及比较分析
BMC Genomics. 2015 Jul 18;16(1):531. doi: 10.1186/s12864-015-1697-8.
8
Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis.嗜线虫致病杆菌(Photorhabdus temperata)基因组解析及转座子突变体文库构建以鉴定在致病过程中发生改变的运动突变体
J Bacteriol. 2015 Jul;197(13):2201-2216. doi: 10.1128/JB.00197-15. Epub 2015 Apr 27.
9
Role of secondary metabolites in establishment of the mutualistic partnership between Xenorhabdus nematophila and the entomopathogenic nematode Steinernema carpocapsae.次生代谢产物在嗜线虫致病杆菌与昆虫病原线虫小卷蛾斯氏线虫互利共生关系建立中的作用
Appl Environ Microbiol. 2015 Jan;81(2):754-64. doi: 10.1128/AEM.02650-14. Epub 2014 Nov 14.
10
The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life.磷酸泛酰巯基乙胺转移酶:一种对生命至关重要的翻译后修饰的催化酶。
Nat Prod Rep. 2014 Jan;31(1):61-108. doi: 10.1039/c3np70054b.

本文引用的文献

1
Mass Production of the Entomogenous Nematode Heterorhabditis heliothidis (Nematoda: Heterorhabditidae) on Artificial Media.在人工培养基上大规模生产昆虫病原线虫嗜菌异小杆线虫(线虫纲:异小杆线虫科)
J Nematol. 1981 Oct;13(4):467-9.
2
Colonial and Cellular Polymorphism in Xenorhabdus luminescens.发光杆菌的殖民与细胞多态性。
Appl Environ Microbiol. 1989 May;55(5):1136-43. doi: 10.1128/aem.55.5.1136-1143.1989.
3
A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates.一种检测微生物脂解活性的简单方法以及关于细胞与脂肪底物之间接触影响的一些观察结果。
Antonie Van Leeuwenhoek. 1957;23(1):15-22. doi: 10.1007/BF02545855.
4
Modular Peptide Synthetases Involved in Nonribosomal Peptide Synthesis.参与非核糖体肽合成的模块化肽合成酶。
Chem Rev. 1997 Nov 10;97(7):2651-2674. doi: 10.1021/cr960029e.
5
Pathogenicity, development, and reproduction of Heterorhabditis bacteriophora and Steinernema carpocapsae under axenic in vivo conditions.无菌体内条件下嗜菌异小杆线虫和小卷蛾斯氏线虫的致病性、发育及繁殖
J Invertebr Pathol. 2000 Jan;75(1):55-8. doi: 10.1006/jipa.1999.4900.
6
Initiation, elongation, and termination strategies in polyketide and polypeptide antibiotic biosynthesis.
Curr Opin Chem Biol. 1999 Oct;3(5):598-606. doi: 10.1016/s1367-5931(99)00015-0.
7
Fimbriae-mediated host-pathogen cross-talk.菌毛介导的宿主-病原体相互作用。
Curr Opin Microbiol. 1998 Feb;1(1):75-81. doi: 10.1016/s1369-5274(98)80145-8.
8
Binding of the fur (ferric uptake regulator) repressor of Escherichia coli to arrays of the GATAAT sequence.大肠杆菌的铁摄取调节因子(fur)阻遏物与GATAAT序列阵列的结合。
J Mol Biol. 1998 Oct 30;283(3):537-47. doi: 10.1006/jmbi.1998.2119.
9
Fimbrial expression in enteric bacteria: a critical step in intestinal pathogenesis.肠道细菌中菌毛的表达:肠道发病机制中的关键步骤。
Trends Microbiol. 1998 Jul;6(7):282-7. doi: 10.1016/s0966-842x(98)01288-8.
10
Purification and characterization of a high-molecular-weight insecticidal protein complex produced by the entomopathogenic bacterium photorhabdus luminescens.昆虫病原细菌发光杆菌产生的一种高分子量杀虫蛋白复合物的纯化与特性分析
Appl Environ Microbiol. 1998 Aug;64(8):3029-35. doi: 10.1128/AEM.64.8.3029-3035.1998.