Alber D, Oberkötter M, Suerbaum S, Claus H, Frosch M, Vogel U
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany.
J Clin Microbiol. 2001 May;39(5):1710-5. doi: 10.1128/JCM.39.5.1710-1715.2001.
We assessed the genetic diversity of 26 Neisseria lactamica strains from epidemiologically related sources, i.e., groups of kindergartens and primary schools in three Bavarian towns, by the partial sequencing of the argF, rho, recA, and 16S ribosomal genes. We found a total of 17 genotypes, of which 12 were found only in one strain. The genotypes comprised 5 alleles of the argF gene, 9 of rho, 8 of recA, and 10 of the 16S ribosomal DNA. Sequence analysis by determination of homoplasy ratios and split decomposition analysis revealed abundant recombination within N. lactamica.
我们通过对argF、rho、recA和16S核糖体基因进行部分测序，评估了来自流行病学相关来源（即巴伐利亚三个城镇的幼儿园和小学群体）的26株乳酸奈瑟菌菌株的遗传多样性。我们共发现了17种基因型，其中12种仅在一株菌株中出现。这些基因型包括argF基因的5个等位基因、rho基因的9个等位基因、recA基因的8个等位基因以及16S核糖体DNA的10个等位基因。通过测定同塑性比率进行的序列分析和分裂分解分析显示，乳酸奈瑟菌内部存在大量重组现象。
