Mechergui Arij, Achour Wafa, Ben Hassen Assia
Service des Laboratoires (UR12ES02), Centre National de Greffe de Moelle Osseuse, Rue Djebel-Lakdhar, Bab Saadoun, 1006, Tunis, Tunisia,
World J Microbiol Biotechnol. 2014 Aug;30(8):2181-8. doi: 10.1007/s11274-014-1637-9. Epub 2014 Mar 18.
We aimed to compare accuracy of genus and species level identification of Neisseria spp. using biochemical testing and 16S rRNA sequence analysis. These methods were evaluated using 85 Neisseria spp. clinical isolates initially identified to the genus level by conventional biochemical tests and API NH system (Bio-Mérieux(®)). In 34 % (29/85), more than one possibility was given by 16S rRNA sequence analysis. In 6 % (5/85), one of the possibilities offered by 16S rRNA gene sequencing, agreed with the result given by biochemical testing. In 4 % (3/85), the same species was given by both methods. 16S rRNA gene sequencing results did not correlate well with biochemical tests.
我们旨在比较使用生化检测和16S rRNA序列分析对奈瑟菌属进行属和种水平鉴定的准确性。使用85株奈瑟菌属临床分离株对这些方法进行了评估，这些分离株最初通过传统生化试验和API NH系统（生物梅里埃公司（®））鉴定到属水平。在34%（29/85）的情况下，16S rRNA序列分析给出了不止一种可能性。在6%（5/85）的情况下，16S rRNA基因测序提供的可能性之一与生化检测结果一致。在4%（3/85）的情况下，两种方法给出了相同的菌种。16S rRNA基因测序结果与生化检测结果相关性不佳。
