Hioki K, Kuramochi T, Endoh S, Terada E, Ueyama Y, Ito M
Central Institute for Experimental Animals, 1430 Nogawa, Kawasaki 216, Japan.
Exp Anim. 2001 Jan;50(1):67-72. doi: 10.1538/expanim.50.67.
BALB/cA mice homozygous for both nu and scid mutations (BALB/cA-nu/nu, scid/scid) were developed by mating between BALB/cA-scid and BALB/cA-nu. These mice have greater longevity than C.B-17-scid because no thymic lymphoma occurs in them unlike in the latter. C.B-17-scid is known to show the leaky phenomenon in which a few clones of functional T and B cells develop in aged C.B-17-scid. Unexpectedly, the leaky B cells and T cells were absent or suppressed in BALB/cA-nu, scid mice when cytokine expressions were determined by RT-PCR, lymphocyte phenotypes by flow cytometry and serum immunoglobulin levels by ELISA. These results indicate that B cell leakiness may be induced by leaked T cells. BALB/cA-nu, scid mice may be useful as a recipient in allo- and xeno-transplantation experiments because of the absence of both thymic lymphomas and leakiness, in addition to lack of hair.
通过将BALB/cA-scid与BALB/cA-nu进行交配，培育出了同时对nu和scid突变纯合的BALB/cA小鼠（BALB/cA-nu/nu，scid/scid）。这些小鼠的寿命比C.B-17-scid更长，因为与后者不同，它们不会发生胸腺淋巴瘤。已知C.B-17-scid会出现渗漏现象，即在老龄C.B-17-scid中会产生一些功能性T细胞和B细胞克隆。出乎意料的是，当通过逆转录聚合酶链反应（RT-PCR）测定细胞因子表达、通过流式细胞术测定淋巴细胞表型以及通过酶联免疫吸附测定（ELISA）测定血清免疫球蛋白水平时，BALB/cA-nu，scid小鼠中不存在或抑制了渗漏的B细胞和T细胞。这些结果表明，B细胞渗漏可能是由渗漏的T细胞诱导的。BALB/cA-nu，scid小鼠除了没有毛发外，由于既不存在胸腺淋巴瘤也不存在渗漏现象，可能在同种异体和异种移植实验中作为受体有用。
