Goodwill R
Genetics. 1975 Feb;79(2):219-29. doi: 10.1093/genetics/79.2.219.
Triple-testcross experiments (Kearsey and Jinks 1968) were employed to investigate the mode of gene action affecting pupa weight in Tribolium castaneum. Their experimental design involves two inbred lines, the F1 progeny and a segregating population derived from the cross of the inbred lines. In the present experiments, four segregating populations were used. These populations included the F2 generation, a select line (SEL) and two relaxed select lines (RSI and RSII). In addition, all possible reciprocal crosses were made among the RSI, RSII, and SEL populations. It was observed that: (1) additive, dominant and epistatic gene effects all made significant contributions to the pupa weight of the progeny from all four segregating populations: (2) there was no evidence of either accumulation of epistasis as a result of selection in the SEL population or decline in epistasis as a result of removing selection pressure from the RSI and RSII populations; and (3) significant negative heterosis and maternal effects contributed to the pupa weight of the crossbred progeny of the RSI, RSII and SEL populations.
采用三重测交实验（Kearsey和Jinks，1968）来研究影响赤拟谷盗蛹重的基因作用模式。他们的实验设计涉及两个近交系、F1代以及由近交系杂交产生的分离群体。在本实验中，使用了四个分离群体。这些群体包括F2代、一个选择系（SEL）和两个放松选择系（RSI和RSII）。此外，在RSI、RSII和SEL群体之间进行了所有可能的正反交。观察到：（1）加性、显性和上位性基因效应均对所有四个分离群体后代的蛹重有显著贡献；（2）没有证据表明SEL群体中因选择导致上位性积累，也没有证据表明RSI和RSII群体中因去除选择压力导致上位性下降；（3）显著的负杂种优势和母体效应影响了RSI、RSII和SEL群体杂交后代的蛹重。
