Robertson K L, Drucker D B, James J, Blinkhorn A S, Hamlet S, Bird P S
Oral Microbiology Laboratory, University of Manchester Dental School, Higher Cambridge Street, Manchester M15 6FH, UK.
J Clin Pathol. 2001 May;54(5):371-6. doi: 10.1136/jcp.54.5.371.
To analyse the microflora of subgingival plaque from patients with Papillon-Lefévre syndrome (PLS), which is a very rare disease characterised by palmar-plantar hyperkeratosis with precocious periodontal destruction.
Bacterial isolates were identified using a combination of commercial identification kits, traditional laboratory tests, and gas liquid chromatography. Some isolates were also subjected to partial 16S rDNA sequencing. Plaque samples were also assayed for the presence of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in a quantitative enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies.
The culture results showed that most isolates were capnophilic and facultatively anaerobic species-mainly Capnocytophaga spp and Streptococcus spp. The latter included S. constellatus, S. oralis, and S. sanguis. Other facultative bacteria belonged to the genera gemella, kingella, leuconostoc, and stomatococcus. The aerobic bacteria isolated were species of neisseria and bacillus. Anaerobic species included Prevotella intermedia, P. melaninogenica, and P. nigrescens, as well as Peptostreptococcus spp. ELISA detected P gingivalis in one patient in all sites sampled, whereas A. actinomycetemcomitans was detected in only one site from the other patient. Prevotella intermedia was present in low numbers.
Patients with PLS have a very complex subgingival flora including recognised periodontal pathogens. However, no particular periodontopathogen is invariably associated with PLS.
分析帕皮永-勒费弗尔综合征（PLS）患者龈下菌斑的微生物群落，PLS是一种非常罕见的疾病，其特征为掌跖角化过度伴早期牙周破坏。
使用商业鉴定试剂盒、传统实验室检测和气相色谱法相结合的方法对细菌分离株进行鉴定。部分分离株还进行了16S rDNA部分测序。还使用单克隆抗体通过定量酶联免疫吸附测定（ELISA）检测菌斑样本中牙龈卟啉单胞菌、中间普氏菌和伴放线放线杆菌的存在情况。
培养结果显示，大多数分离株为嗜二氧化碳菌和兼性厌氧菌，主要是二氧化碳嗜纤维菌属和链球菌属。后者包括星座链球菌、口腔链球菌和血链球菌。其他兼性细菌属于孪生球菌属、金氏菌属、明串珠菌属和口腔球菌属。分离出的需氧菌为奈瑟菌属和芽孢杆菌属的菌种。厌氧菌包括中间普氏菌、产黑色素普氏菌和变黑普氏菌，以及消化链球菌属。ELISA在所有采样部位的一名患者中检测到牙龈卟啉单胞菌，而在另一名患者的仅一个部位检测到伴放线放线杆菌。中间普氏菌数量较少。
PLS患者的龈下菌群非常复杂，包括公认的牙周病原体。然而，没有特定的牙周病原体总是与PLS相关。
