[皱褶假丝酵母产脂肪酶的研究]

[Studies on lipase production from Candida rugosa].

作者信息

Song Q X, Lin J P, Rong Y P, Wei D Z

机构信息

State Key Laboratory of Bioreactor Engineering, Research Institute of Biochemistry, East China University of Science & Technology, Shanghai 200237, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2001 Jan;17(1):101-4.

PMID:11330177

Abstract

We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition. The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source), 0.3% NH4NO3(nitrogen source), 1.2% K2HPO4 and 0.4% MgSO4.7H2O. And the optimal culture condition is initial pH6.5, temperature 30 degrees C, agitation 180 r/min and time 60 h. As a result, and the lipase activity could reach 19.5 u/mL. Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE. The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition. While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5 u/mL within 48 hours.

摘要

我们研究了一些影响皱褶假丝酵母产脂肪酶的因素，这些因素主要包括培养基成分和培养条件。结果表明，产脂肪酶的最佳培养基成分是0.1%葡萄糖、4.0%橄榄油（碳源）、0.3%硝酸铵（氮源）、1.2%磷酸氢二钾和0.4%七水硫酸镁。最佳培养条件为初始pH6.5、温度30℃、搅拌速度180转/分钟、培养时间60小时。结果，脂肪酶活性可达19.5U/mL。同时我们发现表面活性剂有助于脂肪酶的产生，最佳表面活性剂浓度为0.03%甘油聚醚。优化培养基成分和培养条件后，脂肪酶活性提高了170%以上。而在5L发酵罐中，发酵液的脂肪酶活性在48小时内可达33.5U/mL。