School of Chemistry and Biochemistry, Thapar University, India.
J Basic Microbiol. 2010 Feb;50(1):37-42. doi: 10.1002/jobm.200900361.
Extracellular lipase production by Aspergillus sp. (RBD-01) was monitored by modulating pH of the growth medium, ambient temperature for growth, source of nitrogen and percentage of carbon (virgin cottonseed oil). This strain was observed to be viable and produces lipase even up to 50% oil as a main carbon source. Maximum lipase activity of 21.8 U/ml was obtained with 50% (v/v) oil acting as the main carbon source and peptone (0.5% w/v) as nitrogen source. The optimum pH and temperature for enzymatic activity were observed to be 7.5 and 35 degrees C, respectively. The observations are of significance due to limited reports on use of 50% of oil as the main carbon source while obtaining significant lipase activity of 21.8 U/ml.
通过调节生长培养基的 pH 值、环境温度、氮源和碳源（初榨棉籽油）的比例，监测了 Aspergillus sp. (RBD-01) 的细胞外脂肪酶的产生情况。该菌株在高达 50%的油作为主要碳源的情况下仍具有活力并产生脂肪酶。当 50%（体积/体积）的油作为主要碳源，蛋白胨（0.5%重量/体积）作为氮源时，脂肪酶活性达到最大值 21.8 U/ml。观察到最适酶活 pH 值和温度分别为 7.5 和 35 摄氏度。由于有关使用 50%的油作为主要碳源同时获得 21.8 U/ml 显著脂肪酶活性的报道有限，因此这些观察结果具有重要意义。
