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用于高效生产脂肪酶的培养基：一次改变一个变量的方法。

Efficient Media for High Lipase Production: One Variable at a Time Approach.

作者信息

Soleymani Safoura, Alizadeh Houri, Mohammadian Hossein, Rabbani Emad, Moazen Fatemeh, MirMohammad Sadeghi Hamid, Shariat Ziaedin Samsam, Etemadifar Zahra, Rabbani Mohammed

机构信息

Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Department of Biology, Faculty of Basic Sciences, University of Isfahan, Isfahan, Iran.

出版信息

Avicenna J Med Biotechnol. 2017 Apr-Jun;9(2):82-86.

PMID:28496947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5410133/
Abstract

BACKGROUND

Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by , culture media compositions were optimized using ″one variable at a time″ strategy.

METHODS

For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated.

RESULTS

Maximum lipase activity was achieved after 24 of incubation with 1.5% of glucose syrup (1600±69.1 ), 1% of fish powder (1238±36.7 ) and olive oil (1407±2.1 ) as low cost carbon and nitrogen sources and substrate, respectively.

CONCLUSION

Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme.

摘要

背景

脂肪酶在广泛的工业领域都有应用。这些酶的工业价格的一个关键决定因素是培养基成分，研究人员一直在对其进行审查。在这项工作中，为了使[具体微生物名称]产生最大量的脂肪酶，采用“一次一个变量”策略对培养基成分进行了优化。

方法

为此，评估了培养基参数，如低成本和高成本的碳源和氮源、底物及培养时间。

结果

分别以1.5%的葡萄糖浆（1600±69.1[酶活性单位]）、1%的鱼粉（1238±36.7[酶活性单位]）和橄榄油（1407±2.1[酶活性单位]）作为低成本碳源、氮源和底物，培养24小时后达到最大脂肪酶活性。

结论

我们的结果表明，使用低成本来源可显著提高脂肪酶活性；这有助于降低脂肪酶在工业应用中的培养基价格。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/4aa92fa48a20/AJMB-9-82-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/5eb291b41060/AJMB-9-82-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/4b1b83041b1f/AJMB-9-82-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/e75c444ee81e/AJMB-9-82-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/992fe5843478/AJMB-9-82-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/eb88aead8489/AJMB-9-82-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/4aa92fa48a20/AJMB-9-82-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/5eb291b41060/AJMB-9-82-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/4b1b83041b1f/AJMB-9-82-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/e75c444ee81e/AJMB-9-82-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/992fe5843478/AJMB-9-82-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/eb88aead8489/AJMB-9-82-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d70d/5410133/4aa92fa48a20/AJMB-9-82-g006.jpg

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