Liu J J, Guo Y, Zheng S P, Zhang M H
College of Chemistry and Chemical Engineering, Central-south University of Technology, Changsha 410082, China.
Sheng Wu Gong Cheng Xue Bao. 2001 Jan;17(1):94-7.
Investigate the influence of culture media to growth and flavonol glycoside synthesis of calli introduced from seedling of Ginkgo biloba. 6 cell lines were selected from calli by hypoxia stress. Among these cell lines the best one TZ-1 which growth index was 4.12 and the flavonol glycoside content was 1.25% in dried cell which was enhanced 257.1% compared with callus. The stability in subcultures was investigated: The average content of flavonol glycoside was 1.25% in dried cells and the growth index was 3.99 during 6 subcultures. Which variation coefficient was separately 0.065 and 0.048. The results show that hypoxia stress is a efficient method to select suspension cell line of higher productivity of flavonol glycoside.
研究培养基对银杏幼苗愈伤组织生长及黄酮醇苷合成的影响。通过缺氧胁迫从愈伤组织中筛选出6个细胞系。其中最佳细胞系TZ-1的生长指数为4.12，干燥细胞中黄酮醇苷含量为1.25%，与愈伤组织相比提高了257.1%。研究了其继代培养的稳定性：在6次继代培养中，干燥细胞中黄酮醇苷的平均含量为1.25%，生长指数为3.99。其变异系数分别为0.065和0.048。结果表明，缺氧胁迫是筛选黄酮醇苷高产悬浮细胞系的有效方法。
