Fan Y, Wang Y, Tan R, Zhang Z
Pharmaceutical Institute of Nanjing University, Nanjing 210093.
Zhongguo Zhong Yao Za Zhi. 1998 May;23(5):267-9, 319.
HPLC methods have been developed for the determination of Ginkgo flavonol glycosides in the Ginkgo biloba leaves obtained from May to November in the fallen leaves and in the leaves obtained from female and male trees. The method includes hydrolysis of the leaves and subsequent quantitative chromatographic assay of the aglycones, followed by calculation of Ginkgo flavonol glycosides content. The result shows that the leaves obtained in May have the highest content of 0.96%, and then the content decreases from May to August, and from August to November remains almost the same, that is 0.5% also. Fallen leaves have the lowest content of 0.44%. The content range is 0.96% to 0.44%. Male trees have higher content of glycosides than female trees.
已经开发出高效液相色谱法来测定从5月至11月收集的银杏落叶以及雌雄银杏树叶片中的银杏黄酮醇苷。该方法包括叶片水解以及随后对苷元进行定量色谱分析,然后计算银杏黄酮醇苷的含量。结果表明,5月采集的叶片含量最高,为0.96%,然后从5月至8月含量下降,从8月至11月含量几乎保持不变,也是0.5%。落叶的含量最低,为0.44%。含量范围为0.96%至0.44%。雄树中的糖苷含量高于雌树。