Pullammanappallil P C, Chynoweth D P, Lyberatos G, Svoronos S A
Department of Chemical Engineering, University of Florida, Gainesville 32611, USA.
Bioresour Technol. 2001 Jun;78(2):165-9. doi: 10.1016/s0960-8524(00)00187-5.
An automatically controlled, glucose-fed, anaerobic digester was deliberately inhibited by addition of phenol. To overcome the phenol inhibition the feed dilution rate was lowered in such a way that the methane yield from glucose was kept the same as that under normal conditions. The concentrations of acetic and butyric acids remained below 100 mg/l, however, propionic acid accumulated to 2,750 mg/l. Phenol apparently inhibited all tropic groups of organisms and it was shown that the propionic acid was formed from the metabolism of phenol. From the nature of the operating strategy, it was deduced that the digester continued to convert all the glucose that was supplied to methane showing that propionic acid accumulation did not inhibit conversion of glucose to methane. Therefore, propionic acid accumulation may be an effect and not a cause of inhibition of the anaerobic digestion process.
一个自动控制的、以葡萄糖为进料的厌氧消化器通过添加苯酚被蓄意抑制。为克服苯酚抑制作用，进料稀释率以这样一种方式降低，即来自葡萄糖的甲烷产量保持与正常条件下相同。乙酸和丁酸的浓度保持在100毫克/升以下，然而，丙酸积累到2750毫克/升。苯酚显然抑制了所有营养类型的生物体，并且表明丙酸是由苯酚的代谢形成的。从操作策略的性质推断，消化器继续将供应的所有葡萄糖转化为甲烷，这表明丙酸积累并未抑制葡萄糖向甲烷的转化。因此，丙酸积累可能是厌氧消化过程抑制的一个结果而非原因。
