采用酶联免疫吸附测定法（ELISA）对尿8-羟基脱氧鸟苷（8-OH-dG）进行定量测定。
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA.
作者信息
Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K
机构信息
Department of Preventive Medicine, St. Marianna University School of Medicine, Kawasaki, Japan. ssaito@marianna-u-ac-jp
出版信息
Res Commun Mol Pathol Pharmacol. 2000;107(1-2):39-44.
In order to establish a quantitative assay for 8-OHdG concentrations in urine, we examined the precision of a test for the recovery of 8-OHdG in urine by using an ELISA method. The coefficient of variation (CV) for assay with incubation in water or air ranged between 7.0% and 8.4% and between 19.2% and 30.6%, respectively. The data by using incubation in water gave higher accuracy than those in air. The recovery rates of 8-OHdG in urine sample ranged from 95 - 114%. Our results indicated that the excellent sensitivity of this ELISA kit by using incubation in water makes its use possible for the determination in urine with a good reproducibility and recovery of 8-OHdG-spiked samples.
为建立尿液中8-羟基脱氧鸟苷（8-OHdG）浓度的定量检测方法，我们采用酶联免疫吸附测定（ELISA）法检测了尿液中8-OHdG回收率检测的精密度。在水中或空气中孵育进行检测的变异系数（CV）分别在7.0%至8.4%和19.2%至30.6%之间。在水中孵育得到的数据比在空气中孵育的数据准确性更高。尿液样本中8-OHdG的回收率在95%至114%之间。我们的结果表明，该ELISA试剂盒在水中孵育具有出色的灵敏度，使其能够用于尿液检测，对添加8-OHdG的样本具有良好的重现性和回收率。
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