Katzenellenbogen E, Kocharova N A, Zatonsky G V, Kübler-Kiełb J, Gamian A, Shashkov A S, Knirel Y A, Romanowska E
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
FEMS Immunol Med Microbiol. 2001 Apr;30(3):223-7. doi: 10.1111/j.1574-695X.2001.tb01574.x.
On the basis of chemical and methylation analyses, one- and two-dimensional (1)H- and (13)C-NMR spectroscopy, including COSY, TOCSY, NOESY and (1)H, (13)C HSQC experiments, a neutral O-specific polysaccharide isolated from Hafnia alvei strain PCM 1223 lipopolysaccharide (LPS) was found to be an alpha-mannan composed of pentasaccharide repeating units having the following structure:-->3)-alpha-D-Manp-(1-->3)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->. Immunoblotting showed a strong cross-reactivity between anti-H. alvei PCM 1223 serum and LPSs of Escherichia coli O9 and Klebsiella pneumoniae O3. The serological relationship of the LPSs of these bacteria is due to the structural identity of their O-specific polysaccharides, though the LPSs differ in their core regions.
基于化学和甲基化分析、一维和二维（1）H - 和（13）C - NMR光谱，包括COSY、TOCSY、NOESY和（1）H、（13）C HSQC实验，从蜂房哈夫尼亚菌PCM 1223脂多糖（LPS）中分离出的一种中性O - 特异性多糖被发现是一种由具有以下结构的五糖重复单元组成的α - 甘露聚糖：→3）-α - D - 甘露糖基 - （1→3）-α - D - 甘露糖基 - （1→2）-α - D - 甘露糖基 - （1→2）-α - D - 甘露糖基 - （1→2）-α - D - 甘露糖基 - （1→。免疫印迹显示抗蜂房哈夫尼亚菌PCM 1223血清与大肠杆菌O9和肺炎克雷伯菌O3的LPS之间有很强的交叉反应性。这些细菌LPS的血清学关系归因于它们O - 特异性多糖的结构同一性，尽管它们的LPS在核心区域有所不同。
