Shimizu S, Yamada K
Histochem J. 1986 Jul;18(7):357-63. doi: 10.1007/BF01675216.
Changes in glycoconjugates of the zona pellucida induced by maturation, ovulation and fertilization of mouse oocytes have been studied by means of light microscopic methods of cytochemistry. These methods consisted of periodic acid-Schiff (PAS), Alcian Blue pH 1.0 and pH 2.5, and peroxidase-labelled lectin diaminobenzidine (PO-LT-DAB) procedures in combination with the digestion technique with neuraminidase. According to the results obtained, glycoconjugates of the zona pellucida of fertilized eggs contained a smaller amount of sulphate groupings than that in ovarian oocytes, whereas their reactions for sialic acid and fucose residues were significantly stronger in intensity in the former, as compared with those in the latter. The cytophysiological significance of such cytochemical changes in glycoconjugates of the zona pellucida has been discussed with special reference to its functional alterations following maturation, ovulation and fertilization.
运用细胞化学的光学显微镜方法，研究了小鼠卵母细胞成熟、排卵和受精过程中透明带糖复合物的变化。这些方法包括过碘酸希夫反应（PAS）、pH 1.0和pH 2.5的阿尔辛蓝染色、过氧化物酶标记凝集素二氨基联苯胺（PO-LT-DAB）程序以及神经氨酸酶消化技术。根据所得结果，受精卵透明带的糖复合物所含硫酸基团比卵巢卵母细胞中的少，而与后者相比，前者对唾液酸和岩藻糖残基的反应强度明显更强。本文特别参照透明带糖复合物在成熟、排卵和受精后的功能改变，讨论了这些细胞化学变化的细胞生理意义。
