Tatum J L, Briner W H, Goodrich J K
Invest Radiol. 1979 Mar-Apr;14(2):185-8. doi: 10.1097/00004424-197903000-00014.
A simplified solid-state enzymatic iodination procedure for routine labeling of unstable pure protein or complex amino acid-containing molecules is presented. The procedure was designed using agarose-bound lactoperoxidase to iodinate human IgG with iodine-125. This method consistently resulted in a labeling efficiency greater than 90% with high stability and undetectable gross structural alterations of the substrate as evaluated by immunodiffusion and electrophoresis. The technique presented is simple, efficient, and may be employed to yield a sterile, pyrogen-free labeled species.
本文介绍了一种用于不稳定纯蛋白质或含复合氨基酸分子常规标记的简化固态酶促碘化方法。该方法采用琼脂糖结合的乳过氧化物酶，用碘-125对人IgG进行碘化。通过免疫扩散和电泳评估，该方法始终能产生大于90%的标记效率，具有高稳定性，且底物无明显的总体结构改变。所提出的技术简单、高效，可用于产生无菌、无热原的标记产物。
