Sağol O, Yörükoğlu K, Sis B, Tuna B, Ozer E, Güray M, Mungan U, Kirkali Z
Department of Pathology, Dokuz Eylül University Faculty of Medicine, Izmir, Turkey.
Urology. 2001 May;57(5):895-9. doi: 10.1016/s0090-4295(01)00905-0.
To investigate the role of angiogenesis in predicting tumor recurrence and its correlation with established clinicopathologic prognostic factors in superficial transitional cell carcinoma of the bladder.
The paraffin sections of 80 superficial papillary transitional cell bladder carcinoma specimens were stained with CD31 antibody to label the vascular endothelium using the standard streptavidin-biotin-immunoperoxidase method. The vascular surface density (VSD) equivalent to the vascular surface area per unit of tissue volume and number of vessels per square millimeter of stroma (NVES) were assessed by means of stereology, and these morphometric parameters of angiogenesis were statistically analyzed to interpret the relation to tumor recurrence in addition to tumor stage, grade, size, and number and the presence of carcinoma in situ.
VSD and NVES values showed no statistically significant difference between pTa and pT1 tumors or patients with and without recurrence. In contrast, VSD and NVES values were found to increase in higher grade tumors (P = 0.019). VSD values were also higher in patients with coexisting carcinoma in situ in pTa tumors (P <0.001). Tumor number and size and recurrence number and time to the first recurrence did not correlate with any vascular parameters.
Stereologic assessment of angiogenesis does not help to predict recurrence in superficial bladder cancer. Angiogenic parameters appeared to be well correlated with the conventional histologic grading system. Otherwise, the present study did not show any correlation of angiogenesis with any potential prognostic factors. This may be due to the diverse angiogenic pathways occurring in invasive and superficial tumors.
探讨血管生成在预测膀胱浅表性移行细胞癌肿瘤复发中的作用及其与既定临床病理预后因素的相关性。
采用标准链霉亲和素-生物素-免疫过氧化物酶法，用CD31抗体对80例膀胱浅表乳头状移行细胞癌标本的石蜡切片进行染色，以标记血管内皮。通过体视学评估相当于单位组织体积血管表面积的血管表面密度（VSD）和每平方毫米基质中的血管数量（NVES），除了肿瘤分期、分级、大小、数量和原位癌的存在情况外，还对这些血管生成的形态学参数进行统计分析，以解释其与肿瘤复发的关系。
pTa和pT1肿瘤之间或复发患者与未复发患者之间的VSD和NVES值无统计学显著差异。相比之下，发现高级别肿瘤的VSD和NVES值增加（P = 0.019）。pTa肿瘤中并存原位癌的患者VSD值也更高（P <0.001）。肿瘤数量和大小以及复发次数和首次复发时间与任何血管参数均无相关性。
血管生成的体视学评估无助于预测浅表性膀胱癌的复发。血管生成参数似乎与传统组织学分级系统密切相关。此外，本研究未显示血管生成与任何潜在预后因素之间存在任何相关性。这可能是由于侵袭性和浅表性肿瘤中存在多种血管生成途径。
