Guiha R, el Khodeiry S, Mota L, Caffesse R
Department of Stomatology, Division of Periodontics, University of Texas, Houston Dental Branch, Houston, TX, USA.
J Periodontol. 2001 Apr;72(4):470-8. doi: 10.1902/jop.2001.72.4.470.
The goal of this investigation was to histologically evaluate the healing and revascularization of the subepithelial connective tissue graft in dogs.
Six beagle dogs were used in this study. Recession defects were surgically created on maxillary left P2 and right P2, and maxillary and mandibular left central and lateral incisors. The defects were left untreated for 35 days. After elevating a split thickness flap, the graft was harvested from the palate and placed over the denuded root surfaces. The flap was then coronally repositioned and sutured. Three dogs provided the specimens for 7- and 14-day time points and 3 dogs for 28- and 60-day time points. The animals were sacrificed and the blocks obtained were divided in half. One half was processed following the Spalteholtz method and the other was processed for routine histologic examination.
At 7 days, a clot was present at the demarcation zones and it was more organized at 14 days. At 28 days, the junctional epithelium was formed and the demarcation zones could not be delineated. At 60 days, the oral epithelium had regained its normal appearance. The attachment of the graft to the root surface was mediated by a combination of epithelial downgrowth and connective tissue attachment. Minimal new bone and cementum formation was observed. The vascularization of the graft at 7 days originated from the periodontal plexus and the overlying flap. At 14 days, the graft was completely vascularized. At 28 and 60 days, normal vascularization was present.
The vascularization of the connective tissue graft originates from the periodontal plexus, the supraperiosteal plexus, and the overlying flap. The attachment of the graft to the root surface appears to be mediated by a combination of epithelial downgrowth and connective tissue attachment. There is little potential for new cementum and new bone formation.
本研究的目的是从组织学角度评估犬龈下结缔组织移植片的愈合和血管再生情况。
本研究使用了6只比格犬。在上颌左侧P2和右侧P2以及上颌和下颌左侧中切牙和侧切牙上手术制造退缩性缺损。缺损处不做处理35天。掀起一个断层皮瓣后，从腭部获取移植片并置于暴露的牙根表面。然后将皮瓣向冠方重新定位并缝合。3只犬提供7天和14天时间点的标本，3只犬提供28天和60天时间点的标本。处死动物后，将获取的组织块切成两半。一半按照斯帕尔托兹方法处理，另一半进行常规组织学检查。
7天时，分界区出现血凝块，14天时血凝块更有组织性。28天时，结合上皮形成，分界区无法区分。60天时，口腔上皮恢复正常外观。移植片与牙根表面的附着是由上皮向下生长和结缔组织附着共同介导的。观察到极少的新骨和牙骨质形成。7天时移植片的血管化源自牙周丛和覆盖的皮瓣。14天时，移植片完全血管化。28天和60天时，血管化正常。
结缔组织移植片的血管化源自牙周丛、骨膜上丛和覆盖皮瓣。移植片与牙根表面的附着似乎是由上皮向下生长和结缔组织附着共同介导的。新牙骨质和新骨形成的可能性很小。
