Naidong W, Shou W, Chen Y L, Jiang X
Covance Laboratories, Inc., Madison, WI 53704, USA.
J Chromatogr B Biomed Sci Appl. 2001 Apr 25;754(2):387-99. doi: 10.1016/s0378-4347(01)00021-4.
Use of silica stationary phase and aqueous-organic mobile phases could significantly enhance LC-MS-MS method sensitivity. The LC conditions were compatible with MS detection. Analytes with basic functional groups were eluted with acidic mobile phases and detected by MS in the positive ion mode. Analytes with acid functional groups were eluted with mobile phases at neutral pH and detected by MS in the negative ion mode. Analytes poorly retained on reversed-phase columns showed good retention on silica columns. Compared with reversed-phase LC-MS-MS, 5-8-fold sensitivity increases were observed for basic polar ionic compounds when using silica columns and aqueous-organic mobile phase. Up to a 20-fold sensitivity increase was observed for acidic polar ionic compounds. Silica columns and aqueous-organic mobile phases were used for assaying nicotine, cotinine, and albuterol in biological fluids.
使用硅胶固定相和水-有机流动相可显著提高液相色谱-串联质谱（LC-MS-MS）方法的灵敏度。液相色谱条件与质谱检测兼容。具有碱性官能团的分析物用酸性流动相洗脱，并以正离子模式通过质谱检测。具有酸性官能团的分析物用中性pH的流动相洗脱，并以负离子模式通过质谱检测。在反相柱上保留较差的分析物在硅胶柱上显示出良好的保留。与反相LC-MS-MS相比，使用硅胶柱和水-有机流动相时，碱性极性离子化合物的灵敏度提高了5至8倍。对于酸性极性离子化合物，灵敏度提高了20倍。硅胶柱和水-有机流动相用于测定生物流体中的尼古丁、可替宁和沙丁胺醇。
