Vicente M, Pedro M A, Torrontegui G, Cánovas J L
Mol Cell Biochem. 1975 Apr 30;7(1):59-64. doi: 10.1007/BF01732164.
The uptake of glucose and gluconate is under inductive control in Pseudomonas putida. Glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. Glucose and gluconate uptake obeyed saturation kinetics: the apparent Km for glucose was 6 mM and that for gluconate was 0.5 mM. Therefore, transport of both substrates appears to be mediated by enzyme-like carriers. Glucose and gluconate are parallel inhibitors for their uptake9 Strains selected for their inability totransport glucose were found to be deficient in gluconate uptake. The reverse was alsotrue: mutations affecting gluconate entry also blocked the uptake of glucose. These results demonstrate that a common carrier is involved in the uptake of both glucose and gluconate by P. putida cells.
恶臭假单胞菌中葡萄糖和葡萄糖酸盐的摄取受诱导控制。葡萄糖、葡萄糖酸盐和2-酮葡萄糖酸盐都是这些转运能力的良好营养诱导剂。葡萄糖和葡萄糖酸盐的摄取遵循饱和动力学：葡萄糖的表观Km为6 mM，葡萄糖酸盐的表观Km为0.5 mM。因此，两种底物的转运似乎都是由类酶载体介导的。葡萄糖和葡萄糖酸盐是其摄取的平行抑制剂。筛选出的不能转运葡萄糖的菌株被发现缺乏葡萄糖酸盐摄取能力。反之亦然：影响葡萄糖酸盐进入的突变也会阻断葡萄糖的摄取。这些结果表明，恶臭假单胞菌细胞摄取葡萄糖和葡萄糖酸盐涉及一种共同的载体。
