Vicente M
Arch Microbiol. 1975;102(2):163-6. doi: 10.1007/BF00428362.
Fructose transport was not apparently affected in a number of Pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. Fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturation kinetics (apparent Km equal 0.3 mM). The fructose uptake system serves to transport glucose in addition to fructose. The entry of fructose into P.putida cells appears to be mediated also by the glucose-gluconate uptake system, as shown by the ability to accumulate fructose of wild type cells grown on glucose, a substrate that induces the glucose-gluconate uptake system but not the fructose uptake system. In addition, fructose was found to be an inducer of the glucose-gluconate uptake system. The physiological significance of these observations is not clear because the fructose uptake system can provide the cell with a high enough internal concentration of fructose to support maximum growth rate on this hexose, as shown by following the growth course of a glucose-gluconate uptake mutants on fructose.
在一些普通葡萄糖 - 葡萄糖酸盐摄取系统活性紊乱的恶臭假单胞菌菌株中，果糖转运显然未受影响，这表明存在一个独立的果糖摄取系统。葡萄糖 - 葡萄糖酸盐摄取突变体对果糖的摄取由果糖诱导，并遵循饱和动力学（表观 Km 等于 0.3 mM）。果糖摄取系统除了转运果糖外，还能转运葡萄糖。果糖进入恶臭假单胞菌细胞似乎也由葡萄糖 - 葡萄糖酸盐摄取系统介导，这一点可通过在葡萄糖上生长的野生型细胞积累果糖的能力得到证明，葡萄糖是诱导葡萄糖 - 葡萄糖酸盐摄取系统而非果糖摄取系统的底物。此外，发现果糖是葡萄糖 - 葡萄糖酸盐摄取系统的诱导剂。这些观察结果的生理学意义尚不清楚，因为果糖摄取系统可为细胞提供足够高的细胞内果糖浓度，以支持在这种己糖上的最大生长速率，这一点通过跟踪葡萄糖 - 葡萄糖酸盐摄取突变体在果糖上的生长过程得以证明。
