Wong B J, Chao K K, Kim H K, Chu E A, Dao X, Gaon M, Sun C H, Nelson J S
Beckman Laser Institute and Medical Clinic, University of California-Irvine 92612, USA.
Am J Rhinol. 2001 Mar-Apr;15(2):109-16. doi: 10.2500/105065801781543790.
Interest in reconstruction and modification of the facial cartilaginous frameworks using advanced technology and instrumentation is growing rapidly. Despite this maturing interest, no animal model has been established to provide morphologic cartilage tissue with similar characteristics to human septum in suitable quantities. The objective of this study was to characterize porcine and lagomorph (rabbit) nasal septal cartilage tissue. Both models share great similarity with their human counterpart and provide a low-cost, high-volume, and easily obtained source of bulk cartilage tissue. We present a technique for harvesting intact septal cartilages from these species, and characterize select cellular, metabolic, and physical properties using pulse-chase radiolabeling, flow cytometry, and mechanical analysis. Our selective evaluation of key tissue properties establishes these species as appropriate animal models for nasal septal cartilaginous surgery.
利用先进技术和器械对面部软骨框架进行重建和改造的兴趣正在迅速增长。尽管有这种日益成熟的兴趣，但尚未建立起能提供数量合适、具有与人类鼻中隔相似特征的形态学软骨组织的动物模型。本研究的目的是对猪和兔（兔形目动物）的鼻中隔软骨组织进行特征描述。这两种模型与人类鼻中隔有很大的相似性，并提供了一种低成本、大量且易于获取的大块软骨组织来源。我们展示了一种从这些物种中获取完整鼻中隔软骨的技术，并使用脉冲追踪放射性标记、流式细胞术和力学分析来表征选定的细胞、代谢和物理特性。我们对关键组织特性的选择性评估确定了这些物种是鼻中隔软骨手术合适的动物模型。
