Mattioli-Belmonte M, Sampalmieri F, Gabbanelli F, Principato G, Biagini G, Dolci G
CIBAD-Centro Biomateriali Innovativi Istituto di Morfologia Umana Normale, Università degli Studi, Ancona.
Minerva Stomatol. 2000 Nov-Dec;49(11-12):511-20.
Although fluoride has been used for decades either systemically or topically to prevent dental caries, the cellular and molecular mechanisms underlying its action are poorly understood.
An in vitro study of the human keratinocyte cell line NCTC 2544 was conducted in the presence of two different fluoride-containing commercial compounds (Zymafluor and Elmex) to investigate their toxicity threshold and the sequence of events involved in fluoride ion toxicity in this cell population. The toxicity threshold was determined by incubating cells with rising concentrations of Zymafluor and Elmex for 20 h. The study of the sequence of events involved in ion toxicity was performed through a time-effect study by exposing cells to 4 mM fluoride ions and testing them at 2, 6, and 20 h. Cell viability and ultrastructural parameters were assessed: degree of confluence, semiquantitative assessment of dead cells and debris in the supernatant, and morphology.
Ultrastructural morphological analysis showed different cell behaviours with the two compounds; moreover, their toxic effect appeared to be both concentration- and time-dependent.
These data underline the susceptibility of the intracellular communication system to fluoride and show that exceeding the therapeutic dose of fluoride involves substantial risk of toxicity.
尽管氟化物已被系统或局部使用数十年以预防龋齿，但其作用的细胞和分子机制仍知之甚少。
在两种不同的含氟商业化合物（Zymafluor和Elmex）存在的情况下，对人角质形成细胞系NCTC 2544进行了体外研究，以调查其毒性阈值以及该细胞群体中氟离子毒性所涉及的事件顺序。通过用浓度不断增加的Zymafluor和Elmex孵育细胞20小时来确定毒性阈值。通过时间效应研究来进行离子毒性所涉及事件顺序的研究，将细胞暴露于4 mM氟离子中，并在2、6和20小时进行测试。评估细胞活力和超微结构参数：汇合度、上清液中死细胞和碎片的半定量评估以及形态。
超微结构形态分析显示两种化合物具有不同的细胞行为；此外，它们的毒性作用似乎既依赖于浓度又依赖于时间。
这些数据强调了细胞内通讯系统对氟化物的敏感性，并表明超过氟化物治疗剂量会带来很大的毒性风险。
