Langness U, Schors R, Prange E, Lescow R
Z Rheumatol. 1978 Nov-Dec;37(11-12):395-404.
L 929 mouse fibroblasts were cultured as monolayers with different amounts of sodium fluoride, corresponding to those used therapeutically. Proliferation of the cells was inhibited in the logarithmic phase of cell growth. There was no change in the protein concentration in the cells, but the concentration of hydroxyproline containing substances in the cells increased while that of hexuronic acid containing substances decreased. The changes in proliferation and in the concentrations of hydroxyproline containing substances and hexuronic acid containing substances were statistically significant. Although the findings cannot be explained in detail it can be concluded that at therapeutic concentrations sodium fluoride has a definite action in contact with the fibroblast, the typical connective tissue cell. This dose dependant action corroborates clinical experience.
将L929小鼠成纤维细胞培养成单层，添加不同量的氟化钠，其用量与治疗用量相当。在细胞生长的对数期，细胞增殖受到抑制。细胞内蛋白质浓度没有变化，但含羟脯氨酸物质的浓度增加，而含己糖醛酸物质的浓度降低。细胞增殖以及含羟脯氨酸物质和含己糖醛酸物质浓度的变化具有统计学意义。虽然这些发现无法详细解释，但可以得出结论，在治疗浓度下，氟化钠与典型的结缔组织细胞——成纤维细胞接触时具有明确作用。这种剂量依赖性作用证实了临床经验。
