Kim S U
Histochemistry. 1976 Aug 25;48(3):205-17. doi: 10.1007/BF00497456.
Explants of 10--12 day chick embryo spinal cord were cultured by coverslip-roller tube method for 3-80 days. The cellular and subcellular localization of acetylcholinesterase activity in cultured neurons was studied by the thiocholine techniques of Karnovsky and Roots and Lewis and Shute. At the light microscopic level, acetylcholinesterase was demonstrated in the neurons of both ventral and dorsal horn regions. Occasionally neurons migrated in the outgrowth zone exhibited strong intracellular activity. At the electron microscopic level, acetylcholinesterase activity was found in the nuclear envelope, granular endoplasmic reticulum and the Golgi apparatus of the neurons. No enzyme reaction was detected in the glial cell cytoplasm.
采用盖玻片-滚管法将10 - 12日龄鸡胚脊髓外植体培养3 - 80天。运用卡诺夫斯基和鲁茨以及刘易斯和舒特的硫代胆碱技术研究了培养神经元中乙酰胆碱酯酶活性的细胞和亚细胞定位。在光学显微镜水平，乙酰胆碱酯酶在腹角和背角区域的神经元中均有显示。偶尔在生长区迁移的神经元表现出强烈的细胞内活性。在电子显微镜水平，在神经元的核膜、颗粒内质网和高尔基体中发现了乙酰胆碱酯酶活性。在神经胶质细胞胞质中未检测到酶反应。
