Hancock H H, Sigurdsson A, Trope M, Moiseiwitsch J
Department of Endodontics, University of North Carolina School of Dentistry, NC, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 May;91(5):579-86. doi: 10.1067/moe.2001.113587.
The purpose of this study was to determine the composition of the microbial flora present in teeth after the failure of root canal therapy in a North American population. These results were then compared with those of the previous Scandinavian studies.
Fifty-four root-filled teeth with persistent periapical radiolucencies were selected for retreatment. After removal of the root-filling material, the canals were sampled with paper points, and by reaming of the apical dentin. Both samples were grown under aerobic and strict anaerobic conditions. Then the bacterial growth was analyzed.
The microbial flora was mainly of 1 to 2 strains of predominantly gram-positive organisms. Enterococcus faecalis was the most commonly recovered bacterial species.
Bacteria were cultivated in 34 of the 54 teeth examined in the study. E faecalis was identified in 30% of the teeth with a positive culture.
本研究的目的是确定北美人群根管治疗失败后牙齿中存在的微生物菌群组成。然后将这些结果与之前斯堪的纳维亚的研究结果进行比较。
选择54颗有持续性根尖周透射区的根管充填牙进行再治疗。去除根管充填材料后,用纸尖对根管进行取样,并通过扩锉根尖牙本质取样。两个样本均在需氧和严格厌氧条件下培养。然后对细菌生长情况进行分析。
微生物菌群主要为1至2株革兰氏阳性菌。粪肠球菌是最常分离出的细菌种类。
在本研究检查的54颗牙齿中,有34颗培养出细菌。在培养阳性的牙齿中,30%鉴定出粪肠球菌。