[科特迪瓦溃疡分枝杆菌皮肤感染中渗出物显微镜检查的价值与局限性]

[Value and limits of microscopy of exudates in Mycobacterium ulcerans cutaneous infection in Côte d'Ivoire].

作者信息

N'Guessan K, Kouassi Y, Bouzid S, Ehuie P, Koffi K, Oniangue C, Aka N, Dosso M

机构信息

Laboratoire de bactériologie-virologie de l'Institut Pasteur, 01BP 490, Abidjan 01, Côte d'Ivoire.

出版信息

Bull Soc Pathol Exot. 2001 Mar;94(1):9-10.

PMID:11346987

Abstract

The objective of this study was to estimate the interest and the limits of skin exudate microscopy for patients with chronic ulceration with clinically suspected Buruli's ulcer and living in an endemic area in Côte d'Ivoire. Two stained smears, one with Ziehl-Neelsen and the other Dugomier staining, were produced from 140 samples obtained after a swab of skin lesions. The positive smear rate for the acid fast bacilli (AFB) was respectively 16.4% and 12.9% for Dugomier and Ziehl-Neelsen staining. The ulceration with less than one year of evolution have high AFB rates of 15.8% for Ziehl-Neelsen staining and 21.1% for Dugomier's. Microscopic examination of skin exudate in face of ulceration, clinically suspected to be a Buruli's ulcer is not the best tool for biological diagnosis, due to poor technical sensibility. Nevertheless, it remains a good first means of investigation in an endemoepidemic area.

摘要

本研究的目的是评估在科特迪瓦一个地方病流行区，对临床疑似布鲁里溃疡的慢性溃疡患者进行皮肤渗出物显微镜检查的意义及局限性。从140份皮肤病变拭子样本中制备了两张染色涂片，一张用齐尔-尼尔森染色法，另一张用杜戈米耶染色法。杜戈米耶和齐尔-尼尔森染色法检测抗酸杆菌（AFB）的涂片阳性率分别为16.4%和12.9%。病程小于一年的溃疡，齐尔-尼尔森染色法的AFB阳性率为15.8%，杜戈米耶染色法为21.1%。对于临床疑似布鲁里溃疡的溃疡患者，由于技术敏感性较差，皮肤渗出物显微镜检查并非生物学诊断的最佳工具。然而，在地方病流行区，它仍然是一种很好的初步检查方法。